Real-Time PCR

Learn how to detect and quantify nucleic acids in real-time

What you’ll learn:

• Principles of fluorescence detection (SYBR Green, TaqMan)

• qPCR vs conventional PCR

• Setting up standard curves and quantification

• Ct/Cq values interpretation

• Avoiding false positives/negatives

• Efficiency, R², and melt curve analysis

• Troubleshooting low amplification or non-specificity

Who is it for?

🔹 Clinical labs, diagnostic labs, research teams using RT-PCR for gene expression, pathogens, SNPs, etc.

🕒 Duration: 3 hours

📜 Certificate: Yes – completion & skills certificate

🧪 Includes: downloadable protocols + virtual qPCR simulation